EpiGentek – Assay Kits, Antibodies

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What’s DNA Methylation?

DNA methylation occurs by the covalent addition of a methyl group (CH3) on the 5-carbon of the cytosine ring by DNA methyltransferases (DNMTs), resulting in 5-methylcytosine (5-mC). When a CpG island throughout the promoter space of a gene is methylated, expression of the gene is repressed. Various human diseases akin to most cancers occur when DNA methylation won’t be appropriately established and/or maintained.

PRINCIPLE & PROCEDURE

The EpiQuik  Circulating Full Histone H3 Quantification Bundle (Colorimetric) is designed for measuring complete histone H3 in plasma or serum. In an assay with this tools, the histone H3 proteins throughout the plasma/serum sample are captured on the strip wells coated with anti-histone H3 antibody. The captured histone H3 can then be acknowledged with detection antibody adopted by a shade enchancment reagent. The ratio of histone H3 is proportional to the depth of absorbance. Completely the amount of H3 may be quantitated by evaluating to the standard administration.

 

 

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DNA methylation is a dynamic and reversible course of that may result in a lot of intermediate modification varieties. 5-mC reworked from cytosine by DNMTs may be hydroxylated to 5-hydroxymethylcytosine (5-hmC) by TET enzymes. 5-hmC is further modified to 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC) by the similar TET enzymes. Analysis of DNA methylation is essential to understanding and concentrating on DNA methylation–associated changes, which could help decide DNA methylation markers and their natural regulation.

Which DNA Methylation Machine is Correct for Your Experiment?

There are a selection of methods accessible for profiling genome-wide and region-specific DNA methylation. Although worth, minimal sample enter requirements, accuracy, rapidity, and throughput are important issues, choosing the right approach is most important for the success of the analysis.

Sooner than you begin, we advise reviewing any literature that exists related to your proposed experiment. If totally different researchers have already achieved the legwork, you might as properly reap the advantages of this accessible data as a base to assemble your analysis spherical. You and your crew can then make use of variations and distinctive angles to make the analysis completely totally different and attention-grabbing, reviewing the beforehand achieved work fastidiously to find out potential obstacles or points that the sooner researchers encountered.

For those who’re undecided which approach will work in your analysis, we advise starting with a simple pre-screen of worldwide DNA methylation and hydroxymethylation standing in your samples. 1) With the utilization of a fast world DNA methylation or hydroxymethylation assay, you’ll assess whether or not or not your sample accommodates 5-mC or 5-hmC and the way in which lots. Then you’ll resolve the next step and which approach is suitable to utilize. 2) For individuals who uncover your sample accommodates every 5-mC and 5-hmC, oxidative bisulfite-sequencing could very nicely be used.

 

This method can decide and distinguish 5-mC from 5-hmC on a genome-wide scale at single-base resolution. If solely 5-mC is detected with little or no 5-hmC, typical WGBS or RRBS may be carried out. (3) If the sample amount is restricted or single-cell diploma analysis is desired, post-bisulfite sequencing might meet the requirement. (4) For analyzing methylation/hydroxymethylation of specific gene panels akin to a most cancers gene panel or a lot of genomic areas, centered bisulfite-sequencing or centered oxidative bisulfite-sequencing will work.

Methylated DNA Quantification

DNA methylation performs a obligatory operate in common organismal enchancment and cell differentiation in bigger organisms. Gene expression and the occasion of just about all forms of most cancers are moreover tied to DNA methylation. As an illustration, world decrease in 5-methylcytosine content material materials (DNA hypomethylation) might be going introduced on by methyl-deficiency on account of a variety of environmental influences and has been proposed as a molecular marker in a lot of natural processes akin to most cancers. World quantification of DNA methylation is crucial for understanding the roles that gene expression and silencing play in most cancers enchancment and totally different diseases.

 

BOK Antibody

3357-01mg 0.1 mg
EUR 523.7
Description: BOK Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. BOK, a pro-apoptotic Bcl-2 family member, was initially identified in the ovary, and was found to interact with other Bcl-2 family members such as Mcl-1 and Bfl-1. BOK expression is high during early placental development, suggesting that it may also play a role in regulating trophoblast cell proliferation.

BOK Peptide

3357P 0.05 mg
EUR 197.7
Description: (NT) BOK peptide

Bok antibody

20R-1439 100 ug
EUR 781.2
Description: Rabbit polyclonal Bok antibody

BOK Antibody

24253-100ul 100ul
EUR 468

BOK Antibody

ABD3829 100 ug
EUR 525.6

BOK siRNA

20-abx900655
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

BOK siRNA

20-abx909215
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

BOK siRNA

20-abx909216
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

BOK Antibody

1-CSB-PA892353LA01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against BOK. Recognizes BOK from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

BOK Antibody

1-CSB-PA904266
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

BOK Antibody

DF3829 200ul
EUR 420

BOK Antibody

1-CSB-PA437965
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:20-1:100

EP Reagent Biuret Reagent

1011601 1L
EUR 42.18

EP Reagent Iodoplatinate Reagent

1046300 200ML
EUR 360.24

EP Reagent Methoxyphenylacetic Reagent

1053601 100ML
EUR 354.54

EP Reagent Molybdovanadic Reagent

1056700 100ML
EUR 42.18

EP Reagent Phosphomolybdotungstic Reagent

1065000 100ML
EUR 161.88

Automatic CO2 Change Over Unit

INC6176 EACH
EUR 1288.2

Forceps Dissecting Turn Over End

D02129 EACH
EUR 4.67

Gas Change Over Unit 30Psi

GAS1000 EACH
EUR 904.02

Gas Change Over Unit 60Psi

GAS1002 EACH
EUR 905.16

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