EpiGentek – Assay Kits, Antibodies

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What’s DNA Methylation?

DNA methylation occurs by the covalent addition of a methyl group (CH3) on the 5-carbon of the cytosine ring by DNA methyltransferases (DNMTs), resulting in 5-methylcytosine (5-mC). When a CpG island throughout the promoter space of a gene is methylated, expression of the gene is repressed. Various human diseases akin to most cancers occur when DNA methylation won’t be appropriately established and/or maintained.

PRINCIPLE & PROCEDURE

The EpiQuik  Circulating Full Histone H3 Quantification Bundle (Colorimetric) is designed for measuring complete histone H3 in plasma or serum. In an assay with this tools, the histone H3 proteins throughout the plasma/serum sample are captured on the strip wells coated with anti-histone H3 antibody. The captured histone H3 can then be acknowledged with detection antibody adopted by a shade enchancment reagent. The ratio of histone H3 is proportional to the depth of absorbance. Completely the amount of H3 may be quantitated by evaluating to the standard administration.

 

 

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DNA methylation is a dynamic and reversible course of that may result in a lot of intermediate modification varieties. 5-mC reworked from cytosine by DNMTs may be hydroxylated to 5-hydroxymethylcytosine (5-hmC) by TET enzymes. 5-hmC is further modified to 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC) by the similar TET enzymes. Analysis of DNA methylation is essential to understanding and concentrating on DNA methylation–associated changes, which could help decide DNA methylation markers and their natural regulation.

Which DNA Methylation Machine is Correct for Your Experiment?

There are a selection of methods accessible for profiling genome-wide and region-specific DNA methylation. Although worth, minimal sample enter requirements, accuracy, rapidity, and throughput are important issues, choosing the right approach is most important for the success of the analysis.

Sooner than you begin, we advise reviewing any literature that exists related to your proposed experiment. If totally different researchers have already achieved the legwork, you might as properly reap the advantages of this accessible data as a base to assemble your analysis spherical. You and your crew can then make use of variations and distinctive angles to make the analysis completely totally different and attention-grabbing, reviewing the beforehand achieved work fastidiously to find out potential obstacles or points that the sooner researchers encountered.

For those who’re undecided which approach will work in your analysis, we advise starting with a simple pre-screen of worldwide DNA methylation and hydroxymethylation standing in your samples. 1) With the utilization of a fast world DNA methylation or hydroxymethylation assay, you’ll assess whether or not or not your sample accommodates 5-mC or 5-hmC and the way in which lots. Then you’ll resolve the next step and which approach is suitable to utilize. 2) For individuals who uncover your sample accommodates every 5-mC and 5-hmC, oxidative bisulfite-sequencing could very nicely be used.

 

This method can decide and distinguish 5-mC from 5-hmC on a genome-wide scale at single-base resolution. If solely 5-mC is detected with little or no 5-hmC, typical WGBS or RRBS may be carried out. (3) If the sample amount is restricted or single-cell diploma analysis is desired, post-bisulfite sequencing might meet the requirement. (4) For analyzing methylation/hydroxymethylation of specific gene panels akin to a most cancers gene panel or a lot of genomic areas, centered bisulfite-sequencing or centered oxidative bisulfite-sequencing will work.

Methylated DNA Quantification

DNA methylation performs a obligatory operate in common organismal enchancment and cell differentiation in bigger organisms. Gene expression and the occasion of just about all forms of most cancers are moreover tied to DNA methylation. As an illustration, world decrease in 5-methylcytosine content material materials (DNA hypomethylation) might be going introduced on by methyl-deficiency on account of a variety of environmental influences and has been proposed as a molecular marker in a lot of natural processes akin to most cancers. World quantification of DNA methylation is crucial for understanding the roles that gene expression and silencing play in most cancers enchancment and totally different diseases.

 

BOK siRNA

20-abx909216
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

BOK siRNA

20-abx900655
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

BOK Antibody

DF3829 200ul
EUR 304
Description: BOK Antibody detects endogenous levels of total BOK.

BOK Antibody

ABD3829 100 ug
EUR 438

BOK Antibody

3357-002mg 0.02 mg
EUR 171.82
Description: BOK Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. BOK, a pro-apoptotic Bcl-2 family member, was initially identified in the ovary, and was found to interact with other Bcl-2 family members such as Mcl-1 and Bfl-1. BOK expression is high during early placental development, suggesting that it may also play a role in regulating trophoblast cell proliferation.

BOK Antibody

3357-01mg 0.1 mg
EUR 436.42
Description: BOK Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. BOK, a pro-apoptotic Bcl-2 family member, was initially identified in the ovary, and was found to interact with other Bcl-2 family members such as Mcl-1 and Bfl-1. BOK expression is high during early placental development, suggesting that it may also play a role in regulating trophoblast cell proliferation.

BOK Peptide

3357P 0.05 mg
EUR 164.75
Description: (NT) BOK peptide

BOK Peptide

42-325P 0.1 mg
EUR 338
Description: (IN) BOK Peptide

Bok antibody

20R-1439 100 ug
EUR 651
Description: Rabbit polyclonal Bok antibody

Bok antibody

70R-11577 100 ug
EUR 447
Description: Rabbit polyclonal Bok antibody

Bok Antibody

3043-100
EUR 359

HEK-293T Telomerase Over-Expressing Cell Pellet

abx069991-1Pellet 1 Pellet
EUR 398

BOK Polyclonal Antibody

ES9443-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against BOK from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

BOK Polyclonal Antibody

ES9443-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against BOK from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Anti-BOK antibody

STJ118794 100 µl
EUR 277

Anti-BOK antibody

STJ190601 200 µl
EUR 197
Description: Unconjugated Rabbit polyclonal to BOK

Polyclonal Bok Antibody

APR00010G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Bok . This antibody is tested and proven to work in the following applications:

Polyclonal BOK Antibody

APR06350G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BOK . This antibody is tested and proven to work in the following applications:

BOK Polyclonal Antibody

29816-100ul 100ul
EUR 252

BOK Polyclonal Antibody

29816-50ul 50ul
EUR 187

BOK Polyclonal Antibody

ABP57917-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90

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