What’s DNA Methylation?
DNA methylation occurs by the covalent addition of a methyl group (CH3) on the 5-carbon of the cytosine ring by DNA methyltransferases (DNMTs), resulting in 5-methylcytosine (5-mC). When a CpG island throughout the promoter space of a gene is methylated, expression of the gene is repressed. Various human diseases akin to most cancers occur when DNA methylation won’t be appropriately established and/or maintained.
PRINCIPLE & PROCEDURE
The EpiQuik Circulating Full Histone H3 Quantification Bundle (Colorimetric) is designed for measuring complete histone H3 in plasma or serum. In an assay with this tools, the histone H3 proteins throughout the plasma/serum sample are captured on the strip wells coated with anti-histone H3 antibody. The captured histone H3 can then be acknowledged with detection antibody adopted by a shade enchancment reagent. The ratio of histone H3 is proportional to the depth of absorbance. Completely the amount of H3 may be quantitated by evaluating to the standard administration.
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DNA methylation is a dynamic and reversible course of that may result in a lot of intermediate modification varieties. 5-mC reworked from cytosine by DNMTs may be hydroxylated to 5-hydroxymethylcytosine (5-hmC) by TET enzymes. 5-hmC is further modified to 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC) by the similar TET enzymes. Analysis of DNA methylation is essential to understanding and concentrating on DNA methylation–associated changes, which could help decide DNA methylation markers and their natural regulation.
Which DNA Methylation Machine is Correct for Your Experiment?
There are a selection of methods accessible for profiling genome-wide and region-specific DNA methylation. Although worth, minimal sample enter requirements, accuracy, rapidity, and throughput are important issues, choosing the right approach is most important for the success of the analysis.
Sooner than you begin, we advise reviewing any literature that exists related to your proposed experiment. If totally different researchers have already achieved the legwork, you might as properly reap the advantages of this accessible data as a base to assemble your analysis spherical. You and your crew can then make use of variations and distinctive angles to make the analysis completely totally different and attention-grabbing, reviewing the beforehand achieved work fastidiously to find out potential obstacles or points that the sooner researchers encountered.
For those who’re undecided which approach will work in your analysis, we advise starting with a simple pre-screen of worldwide DNA methylation and hydroxymethylation standing in your samples. 1) With the utilization of a fast world DNA methylation or hydroxymethylation assay, you’ll assess whether or not or not your sample accommodates 5-mC or 5-hmC and the way in which lots. Then you’ll resolve the next step and which approach is suitable to utilize. 2) For individuals who uncover your sample accommodates every 5-mC and 5-hmC, oxidative bisulfite-sequencing could very nicely be used.
This method can decide and distinguish 5-mC from 5-hmC on a genome-wide scale at single-base resolution. If solely 5-mC is detected with little or no 5-hmC, typical WGBS or RRBS may be carried out. (3) If the sample amount is restricted or single-cell diploma analysis is desired, post-bisulfite sequencing might meet the requirement. (4) For analyzing methylation/hydroxymethylation of specific gene panels akin to a most cancers gene panel or a lot of genomic areas, centered bisulfite-sequencing or centered oxidative bisulfite-sequencing will work.
Methylated DNA Quantification
DNA methylation performs a obligatory operate in common organismal enchancment and cell differentiation in bigger organisms. Gene expression and the occasion of just about all forms of most cancers are moreover tied to DNA methylation. As an illustration, world decrease in 5-methylcytosine content material materials (DNA hypomethylation) might be going introduced on by methyl-deficiency on account of a variety of environmental influences and has been proposed as a molecular marker in a lot of natural processes akin to most cancers. World quantification of DNA methylation is crucial for understanding the roles that gene expression and silencing play in most cancers enchancment and totally different diseases.
 BOK Peptide |
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3357P |
ProSci |
0.05 mg |
EUR 197.7 |
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Description: (NT) BOK peptide |
 Bok antibody |
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20R-1439 |
Fitzgerald |
100 ug |
EUR 781.2 |
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Description: Rabbit polyclonal Bok antibody |
 BOK Antibody |
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24253-100ul |
SAB |
100ul |
EUR 468 |
BOK Antibody |
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1-CSB-PA892353LA01HU |
Cusabio |
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Description: A polyclonal antibody against BOK. Recognizes BOK from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
BOK Antibody |
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1-CSB-PA904266 |
Cusabio |
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Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100 |
BOK Antibody |
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1-CSB-PA437965 |
Cusabio |
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Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:20-1:100 |
 Bok Antibody |
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3043-100 |
Biovision |
each |
EUR 430.8 |
 BOK Polyclonal Antibody |
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29816-100ul |
SAB |
100ul |
EUR 302.4 |
BOK Polyclonal Antibody |
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29816-50ul |
SAB |
50ul |
EUR 224.4 |
BOK Polyclonal Antibody |
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ABP57917-003ml |
Abbkine |
0.03ml |
EUR 189.6 |
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Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90 |
