Protein A, HHRP Stability Conjugate Reagent

HRP Conjugate Reagent

DESCRIPTION

Software program

Applicable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy, and immunohistochemistry.

Bodily form

chromatographically pure Protein A conjugated to horseradish peroxidase lyophilized from 0.02 M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10 mg/ml BSA- Immunoglobulin and Protease free

Storage and Stability

Lyophilized: Safe for 2 years at -20°C from date of cargo. Rehydrated: Safe for six months at -20°C.

Licensed Knowledge

PROPERTIES

producer/tradename

Upstate®

software program(s)

ELISA: acceptable
dot blot: acceptable
immunohistochemistry: acceptable
western blot: acceptable

shipped in

moist ice

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Till in some other case acknowledged in our catalog or completely different agency documentation accompanying the product(s), our merchandise are meant for evaluation use solely and won’t be to be used for an additional perform, which includes nevertheless should not be restricted to, unauthorized industrial makes use of, in vitro diagnostic makes use of, ex vivo or in vivo therapeutic makes use of or any form of consumption or software program to individuals or animals.
HRP Conjugate Reagent

HRP Conjugate Reagent

Ideas for Use of ab171537 HRP Stability Conjugate Reagent

  • For optimum stability and effectivity, use ab171537 at 100% focus
  • Dilute your HRP conjugate to optimum working focus inside the HRP Stability Conjugate Reagent decision
  • Use diluted conjugate working decision in your assay as common
  • Or, ideally, retailer away from direct delicate at room temperature and use as a stock decision for optimum assay-to-assay consistency

NOTE – When storing any conjugate/ HRP Stability Conjugate Reagent decision for an extended time interval: If a phosphate buffer is used for dilution we propose a remaining focus of no more than 5 mM buffer to avoid the potential of initiating precipitation.

Immediately label your main antibody with HRP

​​​Horseradish peroxidase (HRP) is a 44 kDa glycoprotein with 6 lysine residues, which could possibly be conjugated to antibodies and proteins for use in a variety of functions.

The enzyme label could possibly be visualized through chromogenic reactions. As an example, diaminobenzidine (DAB) inside the presence of hydrogen peroxide (H202) is reworked proper right into a water-insoluble brown pigment. Totally different substrates that may be utilized to measure horseradish peroxidase train embrace ABTS, TMB, and TMBUS.

HRP is a popular detection label utilized in evaluation. Antibody-HRP conjugates are usually utilized in ELISA, IHC, and western blotting. HRP could possibly be conjugated to the primary antibody for direct detection or secondary antibody for indirect detection.

Direct detection is often a preferred methodology inside these functions to avoid cross-species reactivity and to eradicate further laborious wash and separation steps, notably in time-consuming protocols. Nonetheless, immediately conjugating HRP to the antibody or protein of choice could possibly be robust and labor-intensive if using typical methodology.

Our Lightning-Hyperlink HRP antibody labeling gear permits the direct conjugation of your antibody, protein, or peptide to HRP for use in any software program. The gear solely requires 30 seconds of hands-on time, and conjugates are ready to utilize after solely three hours. Furthermore, there is not a need for extra wash or separation steps submit conjugation.

 

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