Immediately label your main antibody with HRP

​​​Horseradish peroxidase (HRP) is a 44 kDa glycoprotein with 6 lysine residues, which could possibly be conjugated to antibodies and proteins for use in a variety of functions.

The enzyme label could possibly be visualized through chromogenic reactions. As an example, diaminobenzidine (DAB) inside the presence of hydrogen peroxide (H₂0₂) is reworked proper right into a water-insoluble brown pigment. Totally different substrates that may be utilized to measure horseradish peroxidase train embrace ABTS, TMB, and TMBUS.

HRP is a popular detection label utilized in evaluation. Antibody-HRP conjugates are usually utilized in ELISA, IHC, and western blotting. HRP could possibly be conjugated to the primary antibody for direct detection or secondary antibody for indirect detection.

Direct detection is often a preferred methodology inside these functions to avoid cross-species reactivity and to eradicate further laborious wash and separation steps, notably in time-consuming protocols. Nonetheless, immediately conjugating HRP to the antibody or protein of choice could possibly be robust and labor-intensive if using typical methodology.

Our Lightning-Hyperlink HRP antibody labeling gear permits the direct conjugation of your antibody, protein, or peptide to HRP for use in any software program. The gear solely requires 30 seconds of hands-on time, and conjugates are ready to utilize after solely three hours. Furthermore, there is not a need for extra wash or separation steps submit conjugation.